Similar surface phenotype of adult and neonatal DCs. (A) CD11b/CD11c density plot of DCs isolated from spleens of adult or neonatal mice (ex vivo) or derived by culturing precursors in GM-CSF or Flt-3L. Acquisition gates were set to exclude debris and dead cells, and quadrants for analysis were set based on control isotype-stained samples. Percentage of positive cells is indicated for each quadrant. (B) B220 expression on neonatal (black filled) or adult (gray line) DCs. Percentage of positive cells for adults (a) or neonates (n) is indicated. (C) H2K^b (MHC-I) and CD86 expression on ex vivo or Flt-3L neonatal and adult DCs after short-term culture (18 hours) without (gray line) or with (black filled) LPS (100 ng/mL) or pI:C (50 μg/mL). The figures show one representative experiment of 3.