LV-mediated GFP expression in GFP transgenic mice. (A) Confocal immunofluorescence analyses of liver sections immunostained for GFP and MHC II from CMV-GFP LV-injected FVB/N TgN (Tie2GFP) transgenic mice that express GFP in endothelial cells. Representative sections from 3 mice were analyzed per time point. Original magnification, × 400. (B) Quantitative real-time PCR of vector DNA from the liver and spleen DNA of LV-injected transgenic mice at different times after injection. Means ± SD, n = 3, are shown. (C) FACS analysis of liver cell suspensions from PBS- and LV-injected transgenic mice labeled with CD4 and CD8 antibodies. The percentage of positive cells in the mononuclear cell gate (mean ± SD, n = 3) is shown.