Role of PAF and its receptors on VEGF-A₁₆₅–induced neutrophil adhesion to HUVECs. HUVECs were pretreated with the indicated PAFR antagonists for 15 minutes prior to the addition of neutrophils (1 × 105) and to the stimulation of confluent HUVEC with VEGF-A₁₆₅ (10^–9 M, 7.5 minutes; A) or PAF (10^–9 M, 7.5 minutes; B). Pretreatment with an intracellular (LAU 8080), an extracellular (BN 52021), and nonspecific PAFR antagonist (CV-3988) reduced significantly the adhesion of neutrophils to HUVECs mediated by VEGF-A₁₆₅ and PAF. Data are means ± SEM of at least 6 experiments; ***P ≤ .001 as compared to DPBS, †††P ≤ .001 compared to VEGF-A₁₆₅ (A) or PAF (B).