Delayed onset but similar phenotype of leukemic disease in mice that received transplants of p190-BCR-ABL-transduced fetal liver from Pik3r1 null embryos. Wild-type and Pik3r1 null fetal liver transduced with p190-BCR-ABL were allowed to expand one week in vitro prior to transplantation of 1 × 106 cells into SCID mice. (A) Kaplan-Meyer plot showing number of days until leukemia development. Of the mice, 4 received wild-type cells (from 2 pooled embryos) and 4 received Pik3r1 null cells (from one embryo). (B) Bone marrow and spleen cells were cytospun and stained with Giemsa/Wright to show the lymphoblastic phenotype. Cells were also analyzed by FACS for expression of GFP, B220, and BP-1. Original magnifications of the panels are shown below the images.
