Statin treatment reduces the nuclear amount of p50-NFκB∼C/EBPβ complexes. Coimmunoprecipitation was performed on nuclear extracts prepared from HuH7 cells and freshly isolated primary human hepatocytes preincubated for 17 hours with 3 μM (HuH7 cells) and 10 μM (primary hepatocytes) simvastatin (Simva), 50 μM Wy 14643 (WY), or vehicle and stimulated with 10 ng/mL IL-1α (HuH7 cells) or 25 ng/mL IL-1α (primary hepatocytes) for 18 hours. Anti-p50-NFκB or anti-MMP-8 control antibody (Control-Ab) was used for immunoprecipitation and C/EBPβ bound to precipitated p50-NFκB was detected by Western blot.