Statin treatment inhibits NFκB activity. (A) HuH7 cells were preincubated with 3 μM simvastatin (Simva) for 24 hours and subsequently were stimulated with 10 ng/mL IL-1α for 18 hours. Western blot analysis of total cellular and nuclear extracts for p50-NFκB and C/EBPβ expression. (B) HuH7 cells were incubated with simvastatin or Wy 14643 (WY) for 24 hours and analyzed for IκBα expression in total cell extracts. Control β-actin and histone H1 expression levels confirm equal loading. (C) HuH7 cells were incubated with Wy 14643 (10 μM, 30 μM, 100 μM), atorvastatin (1 μM, 3 μM, 10 μM), simvastatin (1 μM, 3 μM, 10 μM), transiently transfected with p3xNFκB-luc, and subsequently were stimulated with 10 ng/mL IL-1α for 18 hours. Data represent means ± SD. ^*P < .05 compared with control.