Statin treatment suppresses IL-1-induced huCRP gene expression in human liver cells. (A) Human liver slices were incubated with 5 μM simvastatin (simva), 250 μM Wy 14643 (WY), or vehicle (DMSO; Con) for 24 hours and then stimulated with 25 ng/mL IL-1α for an additional 24 hours. HuCRP was determined in culture medium by ELISA. Data represent means ± SD of 3 experiments. (B) HuH7 cells were treated with Wy 14643 (10 μM, 30 μM, 100 μM), atorvastatin (1 μM, 3 μM, 10 μM), or simvastatin (1 μM, 3 μM, 10 μM), transiently transfected with phuCRP-luc, and subsequently were stimulated with 10 ng/mL IL-1α for 18 hours. Shown data represent means ± SD. ^*P < .05 compared with control.