Functional profile of autoreactive CD4⁺ and CD8⁺ T cells from patients with MS versus healthy subjects. SYBRGreen-based real-time PCR analyses were performed to quantify the expression of indicated molecules in flow-sorted MBP-, PLP-, MOG-, MAG-, or OMGP-specific CD4⁺ (A) and CD8⁺ (B) T cells, sorted from healthy subjects (▪) or patients with MS (). (A) The data represent a total of 10 autoreactive CD4⁺ T-cell populations from healthy subjects and 14 autoreactive CD4⁺ T-cell populations from patients with MS (representing responses to the 5 different CNS antigenic mixes from 3 healthy subjects and 6 patients with MS). (B) The data represent a total of 11 autoreactive CD8⁺ T-cell populations from 3 healthy subjects and 17 autoreactive CD8⁺ T-cell populations from 6 patients with MS. The data are normalized to 2 housekeeping genes (GAPDH and β-actin; assigned a value of 100) and are expressed as the mean relative expression (+ 2 SEM) of the indicated molecules in all autoreactive specimens. Significant P values (< .05) are boxed and borderline values (< .10) are highlighted.