Annexin binding of purified neutrophils from controls, obligate carriers, and BTHS patients in comparison with apoptotic neutrophils. Freshly purified granulocytes from healthy donors (Controls) and granulocytes from the same donor after overnight culture with more than 80% annexin-V binding (Apoptotic) were compared with freshly purified neutrophils from obligate carriers and BTHS patients. The geometric mean fluorescence intensity (geo-MFI) of annexin-V–FITC was determined by flow cytometry; the mean ± SEM is given per experimental cohort of individuals in parentheses.