Cardiolipin composition and spectra of some other phospholipids determined by HPLC-MS in freshly isolated BTHS granulocytes. (A,D,F) Freshly purified control neutrophils; (B) neutrophils from the same donor after overnight culture with more than 80% annexin-V binding; (C,E,G) freshly purified neutrophils from a BTHS patient with more than 60% annexin-V binding. CL molecular species are shown in panels A-C. Arrows indicate the most abundant CL molecular species and their fatty acid composition. BTHS neutrophils did not contain CL species either in fresh cells (C) or in apoptotic cells (not shown). Phosphatidyl inositol molecular species are shown in panels D-E. Phosphatidyl choline and sphingomyelin molecular species are shown in panels F-G. Representative results from 3 BTHS patients and 3 control individuals.