Freshly isolated granulocytes from patients with BTHS display increased annexin-V binding. Freshly purified granulocytes from 7 patients with BTHS and age-matched control individuals (n = 14) were stained by annexin-V–FITC and propidium iodide (PI) and analyzed by flow cytometry. (A) Representative plots for a patient and an age-matched control of the same day; numbers indicate the percentage of annexin-V⁺ cells among total cell population. (B) Summarized data of all measurements performed. Values represent mean ± SD. *P < .05 by the Student t test.