Confirmation of Aurora-B/AIM-1 localization at mitosis. (A) 16N mouse primary megakaryocyte at prophase. Cells were stained with DAPI (blue; i), anti–Aurora-B/AIM-1 antibody (red; ii), and anti–α-tubulin antibody (green; iii), or with triple staining (iv). (B) 8N mouse primary megakaryocyte at prophase. Cells were stained with DAPI (blue; i), anti–Aurora-B/AIM-1 antibody (red; ii), anti-centromere antibody (green; iii), or with triple staining (iv). (C) Mouse primary 16N megakaryocyte at anaphase. Cells were stained with DAPI (blue; i), anti–Aurora-B/AIM-1 antibody (red; ii), or anti–α-tubulin antibody (green; iii). Triple staining (iv). Shown are representative images captured as described in “Materials and methods.” Original magnification, × 1500.