Localization and expression of Aurora-B/AIM-1 at mitosis. Mouse primary megakaryocytes were stained with DAPI (blue; i) or anti–Aurora-B/AIM-1 antibody (red; ii) and were subjected to double staining (iii) or triple staining (iv) with FITC-conjugated anti-CD41 (green). Shown are representative images captured as described in “Materials and methods.” (A) Megakaryocyte with ploidy of 16N at prophase. (B) Megakaryocyte with ploidy of 4N at anaphase. (C) Megakaryocyte with ploidy of 16N at anaphase. (D) Megakaryocyte with ploidy of 16N at anaphase. Original magnification, × 1500.