LPS maturation inhibits C1q production and expression in mDCs. Day-7 immDCs and macrophages were cultured for 48 hours in the presence or absence of LPS. (A) Maturation after culture with LPS was confirmed by increased CD86 expression. To detect the mC1q, the mAb 2204 was used. White histograms indicate isotype control. 48-hour supernatants were tested for C1q (B) and IL-6 (C). Data presented (B-C) are mean ± SD of triplicate analysis in 1 donor (representative of 3 donors). *P < .0001 LPS stimulation compared with culture in normal medium (2-way ANOVA of 3 experiments).