Staining of HIV/HCV-coinfected macrophages for HCV and HIV antigens. (A) Infected macrophages stained with monoclonal antibodies against nonstructural protein 3 (NS3) and visualized with DAB. Two of 5 macrophages shown demonstrate brown granular cytoplasmic staining (original magnification, × 400). Cell shown in the bottom insert is positive for both p24 (left) and NS3 (right). (B) Relationship between HCV RNA cellular load at 2 weeks and the proportion of cells staining for NS3 (r = 0.94; P < .01 by Spearman rank test).