CD200 mRNA expression as DCs undergo apoptosis. (A) CD11c⁺ cells were isolated and cultured in complete DMEM to induce apoptosis. Numeric values denote (B) semiquantitative RT-PCR for CD200 and β-actin. Numbers adjacent to gel represent densitometry values for above spots. (C) Quantitative real-time RT-PCR for CD200 and18S rRNA were performed on mRNA isolated at various times after culture. Results are representative of 3 replicate experiments. ^*Statistically significant from 0 hour (P < .05) by Student t test. Error bars indicate ±1 SD.