Quantitative RT-PCR analysis of genes encoding angiogenic factor. Comparison of mRNA encoding AGF (A), bFGF (B), VEGF (C), VEGF164 (D), VEGF 120 (E), VEGF 188 (F), placental-derived growth factor (PlGF) (G), and Ang-1 (H) from ear tissues of two 8-week-old K14-AGF transgenic mice (lines 3 and 6; ▪) and their littermate controls (control; □). Columns represent mean values ± SD (n = 3). The relative abundance of transcripts was normalized to the constitutive expression level of GAPDH mRNA. *P < .001; **P < .01, relative to controls.