Effects of anti–DC-SIGN mAbs on exogenous presentation of HIV-1 antigens by MHC-I. C1RA2 and C1RA2-DCS cells (upper panels) or primary immature HLA-A2⁺ DCs (lower panels) were pretreated with AZT, exposed to the indicated viruses, and an IFNγ Elispot assay was performed using the anti-Gag CD8⁺ CTL line EM71-1 as effectors. Anti–DC-SIGN (8A5 + 1B10), anti-CD4 (Q4120), and IgG isotype control mAbs (at 20 μg/mL) were added 30 minutes before viral exposure.