IL-6 stimulation induced the phosphorylation of Akt, p70S6K, and FKHR in NOP2 but not in ILKM2 and ILKM3. Phosphorylation of Stat3, ERK1/2, Akt, p70S6K, and FKHR in myeloma cell lines stimulated with IL-6 (2 ng/mL), IGF-I (100 ng/mL), or insulin (100 ng/mL) for 0, 10, or 30 minutes was determined by Western blot analysis using phospho-specific antibodies. Western blots of 3 cell lines by IL-6 stimulation on the same blot (A) and of each cell line by 3 reagents (B) are shown. IL-6 stimulation induced the phosphorylation of Akt, p70S6K, and FKHR in NOP2 but not in ILKM2 and ILKM3, whereas the phosphorylation of ERK1/2, Akt, p70S6K, and FKHR but not of Stat3 in NOP2, ILKM2, and ILKM3 stimulated with IGF-I or insulin was comparable with NOP2 with IL-6.