Enhanced clearance of PIGA^- RBCs by liver macrophages is responsible for their shortened half-life. Wild-type mice received 5 intravenous clodronate-liposome injections (0.01 mL/g) every fourth day. The bold bar below the diagram indicates the time points of injections. PKH-labeled RBCs were injected 2 days after the first injection. The percentage of stained PIGA^- and PIGA+ cells was determined for each time point and normalized to 2 hours after injection time point. Liposome-encapsulated PBS was used as a control. Data represent mean ± SD.