American Society of Hematology

Figure 4.

$Figure 4. Analysis of the HLA restriction of CTL-induced target cell killing using the CFSE-based cytotoxicity assay. The MNC fraction of a patient with AML positive for HLA-A2/HA-1 was stained with CFSE at day -1. At day 0, GM-CSF, IL-3, and G-CSF were added. The target population was incubated in the presence of absence of saturating concentrations of blocking HLA class I or II antibodies before the HLA-A2-restricted HA-1-specific CTL clone was added at a 0.5:1 E/T ratio. Plotted are the calculated absolute numbers of viable CD33+ leukemic cells (± SD) after 24, 48, and 120 hours of CTL incubation.$

Analysis of the HLA restriction of CTL-induced target cell killing using the CFSE-based cytotoxicity assay. The MNC fraction of a patient with AML positive for HLA-A2/HA-1 was stained with CFSE at day -1. At day 0, GM-CSF, IL-3, and G-CSF were added. The target population was incubated in the presence of absence of saturating concentrations of blocking HLA class I or II antibodies before the HLA-A2-restricted HA-1-specific CTL clone was added at a 0.5:1 E/T ratio. Plotted are the calculated absolute numbers of viable CD33⁺ leukemic cells (± SD) after 24, 48, and 120 hours of CTL incubation.

This Feature Is Available To Subscribers Only