![Figure 7. Impaired growth of NPM/ALK-expressing cells in vivo after Bcl-XL down-regulation.(A) Ba/F3-NPM/ALK+ cells (10 × 106) bearing an empty vector control (N/A-pEV, □) and a Dox-inducible ASBcl-XL antisense transgene (N/A-pAS, ○) were injected intravenously into nude mice. Half of the mice from each group (N/A-pEV, ▪; N/A-pAS, •) was maintained under continuous administration of doxycycline (0.2 mg/mL) in the drinking water. Survival of the animals was monitored every other day. (B) Animals injected with N/A-pEV and N/A-pAS cells and bearing measurable nodules (mean tumor weight was 235 mg; 95% confidence interval [CI] = 200-320 mg) were treated with Dox (0.2 mg/mL) in the drinking water. After 3 days of Dox treatment, mice were killed and tumors extracted and analyzed in Western blot (WB) with anti-Bcl-XL and anti-ALK antibodies.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/103/7/10.1182_blood-2003-09-3144/6/zh80070459170007.jpeg?Expires=1724247559&Signature=z70qbo5wRJPRsOF8zcMYpWQfNqO5ONMZ~nh0PeagPv~dUhMLH1feS7sE50tjhlFIqaRabtil-tzctuCqkMcH6ZJd6BQUOi89LUnPAXW0kMwP9mEVpUvmcMdzdq1mKjxE3f1TBwlgxMDG4eWkzzP2q2DhK9plV1NCJjmha5SX69~Y7USPP64cSOVFodyN84v~EQDTHox6d~5vm2DQQD9Pkd~r6NG0QYx36~iUu547D1a-ZltLD28cw9MgOX5Us8vyR-Vx1XFUz9JEer~sEXJUp6hdHj-I2vAU0X0hzrk7uxWM2gDPxzFyScov5MmyFOONeDk7ET-~NFQucLc-6gxf3g__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Impaired growth of NPM/ALK-expressing cells in vivo after Bcl-XL down-regulation.(A) Ba/F3-NPM/ALK+ cells (10 × 106) bearing an empty vector control (N/A-pEV, □) and a Dox-inducible ASBcl-XL antisense transgene (N/A-pAS, ○) were injected intravenously into nude mice. Half of the mice from each group (N/A-pEV, ▪; N/A-pAS, •) was maintained under continuous administration of doxycycline (0.2 mg/mL) in the drinking water. Survival of the animals was monitored every other day. (B) Animals injected with N/A-pEV and N/A-pAS cells and bearing measurable nodules (mean tumor weight was 235 mg; 95% confidence interval [CI] = 200-320 mg) were treated with Dox (0.2 mg/mL) in the drinking water. After 3 days of Dox treatment, mice were killed and tumors extracted and analyzed in Western blot (WB) with anti-Bcl-XL and anti-ALK antibodies.
