Analysis of the in vivo DC reconstitution potential of monocytes after transfer into irradiated recipients. Density plots show the phenotypic analysis of monocyte-derived cells after gating for Ly-5.2⁺ cells, performed on spleen low-density cell fractions, tail vein blood, and thoracic cavity, at the indicated times after monocyte transfer into irradiated recipients. Control mice were injected intravenously with Ly-5.1 recipient-type bone marrow cells. The percentage of Ly-5.2⁺ cells as well as the percentage of cells in each quadrant of the density plots corresponding to the phenotypic analysis is indicated. Data are representative of 4 (spleen) and 2 (tail vein and thoracic cavity) experiments with similar results.