Figure 7.
Effect of LY294002 and PD98059 on the phosphorylation of Akt and ERK. (A) Whole cell lysates were prepared from HUVECs (1 × 106 cells) after treatment with PBS, LY294002 (10 μM), or PD98059 (30 μM) in combination with aggretin (0.1 μM) for 30 minutes or 24 hours. Phosphorylated Akt was detected as described in the legend to Figure 6. (B) HUVECs treated with LY294002 (10 μM) or PD98059 (30 μM) in combination with aggretin (0.1 μM) were cultured for 30 minutes or 24 hours and then lysed and subjected to Western blot (WB) analysis with phosphospecific-ERK1/2. The reprobing with anti-α-tubulin antibody was the internal control. The pattern is a representative one of at least 3 similar results.
