Increase in MCP-1 levels in the lung after allogeneic BMT. Animals received allogeneic (▪) or syngeneic (□) BM transplants as in Figure 1. (A) MCP-1 mRNA expression was determined after BMT by RNAase protection assay. (B) MCP-1 expression is increased after allogeneic BMT compared with syngeneic controls at all time points. (C) BALF protein levels of MCP-1 (ELISA) are increased at week 2 and week 6 after allogeneic BMT. Data are presented as mean ± SEM; n = 3 to 10 animals per group; ^*P < .01. (D-E) Immunohistochemical alkaline phosphatase staining for MCP-1 in lungs from syngeneic (left) and allogeneic (right) BM transplant recipients 14 days after transplantation. Original magnifications: × 200 (D); × 400 (E).