Figure 5.
![Figure 5. Confocal microscopy for calumenin, cyclophilin A, and secretogranin III. (A) Platelets were adhered to fibrinogen-coated slides for 5 (platelets resting) and 60 minutes (platelets activated and spread). A granular staining pattern similar to thrombospondin was observed for cyclophilin A and secretogranin III at both time points, whereas a more diffuse pattern was observed for calumenin. (B) Activated platelets labeled with CD41 and a rhodamine-labeled secondary antibody, and dual stained with antibodies to secretogranin III, cyclophilin A, and calumenin (labeled with fluorescein isothiocyanate [FITC]–conjugated secondary antibodies). (C) Secondary antibodies alone. Original magnification × 63.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/103/6/10.1182_blood-2003-08-2804/6/zh80060458180005.jpeg?Expires=1724241209&Signature=ibew761fY7Wg9vkVZ-4YfVgFFoYsPdU2Lk9LzRLPqwfmPeqwBQaEi2rQ0s23nLmhnx6fiRdmkv565zkS1fnmNeTGyokt-DBRudEm2LRlSJiLhCOiNYY2EnyY1r3rc-9xvf4fotZdgoNX9DsPR5FrCV2Ga1qaXysffxqCDg5IwP2qJssd-AnrXWNJ1wGABNL8jFFSbJAI-a4Lw5R6px0cYU6-Z7JBqCW3WCvO-Jq7YgIR98T0k9kBvhVDhiplEK0iAxX8OonQOlhOQMqE4LH4RkkkOwXrQ389LNvb71UmVTeM9Ze1yPtJzMEmZGQrZ1V7y11xIISPrOIQKp2HnpvAYQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Confocal microscopy for calumenin, cyclophilin A, and secretogranin III. (A) Platelets were adhered to fibrinogen-coated slides for 5 (platelets resting) and 60 minutes (platelets activated and spread). A granular staining pattern similar to thrombospondin was observed for cyclophilin A and secretogranin III at both time points, whereas a more diffuse pattern was observed for calumenin. (B) Activated platelets labeled with CD41 and a rhodamine-labeled secondary antibody, and dual stained with antibodies to secretogranin III, cyclophilin A, and calumenin (labeled with fluorescein isothiocyanate [FITC]–conjugated secondary antibodies). (C) Secondary antibodies alone. Original magnification × 63.
