Western blot for calumenin, cyclophilin A, and secretogranin III. The presence of calumenin (A), cyclophilin 4 (B), and secretogranin III (C) was confirmed in lysates from control (platelet control, PC) and thrombin-activated (platelet activated, PA) platelets, as well as the thrombin-activated releasate (releasate activated, RA). These proteins were not found in the supernatant from unactivated platelets (releasate control, RC). In addition, SgIII and calumenin were not detected in a crude leukocyte lysate (WBC), although cyclophilin 4 was present in low amounts.