Expression of CXCR3 and CXCR4 on Vδ1 and Vδ2 γδ T cells in HIV-1–infected patients. Ex vivo analysis of circulating lymphocytes from HIV-1–infected patients (2 representative phenotypes of 28 patients). (A-D) Patient 6. (E-H) Patient 17. Double immunofluorescence of lymphocytes in the whole blood, with Alexafluor 488–labeled anti-Vδ1 (A,B,E,F) or anti-Vδ2 (C,D,G,H) mAbs compared with PE-conjugated anti-CXCR3 mAb (A,C,E,G) or PE-conjugated anti-CXCR4 mAb (B,D,F,H). Samples were analyzed using FACS gated to lymphocytes, and results are expressed as log green (x-axis) compared with log red (y-axis) fluorescence intensity. Numbers in the quadrants of each panel indicate the percentage of double-positive (upper right) or single-positive (upper left and lower right) cells.