Figure 2.
Figure 2. Expression of CXCR3 and CXCR4 on Vδ1 and Vδ2 γδ T cells in healthy donors. Ex vivo analysis of circulating lymphocytes from healthy donors (2 representative phenotypes of 15 donors): double immunofluorescence of lymphocytes in the whole blood, with Alexafluor 488–labeled anti-Vδ1 (A-C) or anti-Vδ2 (D-F) mAbs compared with PE-conjugated anti-CXCR4 mAb (A,C,D,F) or PE-conjugated anti-CXCR3 mAb (B,E). Samples were analyzed using FACS gated to lymphocytes, and results are expressed as log green (x-axis) compared with log red (y-axis) fluorescence intensity (arbitrary units [au]). Numbers in the quadrants of each panel indicate the percentage of double-positive (upper right) or single-positive (upper left and lower right) cells.

Expression of CXCR3 and CXCR4 on Vδ1 and Vδ2 γδ T cells in healthy donors. Ex vivo analysis of circulating lymphocytes from healthy donors (2 representative phenotypes of 15 donors): double immunofluorescence of lymphocytes in the whole blood, with Alexafluor 488–labeled anti-Vδ1 (A-C) or anti-Vδ2 (D-F) mAbs compared with PE-conjugated anti-CXCR4 mAb (A,C,D,F) or PE-conjugated anti-CXCR3 mAb (B,E). Samples were analyzed using FACS gated to lymphocytes, and results are expressed as log green (x-axis) compared with log red (y-axis) fluorescence intensity (arbitrary units [au]). Numbers in the quadrants of each panel indicate the percentage of double-positive (upper right) or single-positive (upper left and lower right) cells.

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