Compared to control mice, fbg–/– mice form unstable thrombi, and both 7E9-treated and γΔ5 mice form small mural thrombi. Representative recordings of blood flow through the carotid artery before and after FeCl3 injury in mice from each group. Blood flow through the carotid artery was recorded using a Doppler flow probe. Just before the injury, the lubricating jelly covering the probe was aspirated and then the site was rinsed with normal saline and dried. As a result, no blood flow was recorded during this time interval. Injury was induced by application of FeCl3 (arrow; time 0) for 3 minutes, after which the artery was covered with lubricating jelly and flow was continuously recorded for 30 minutes. (A) A control animal that developed an occlusive thrombus at 7.2 minutes. Note that the blood flow remained dramatically reduced thereafter. (B-C) Results from 2 representative fbg–/– mice. The animal in panel B had multiple abrupt episodes of increased carotid blood flow corresponding to extensive embolization. Even after a sustained period of virtually no blood flow, at about 25 minutes embolization recurred and was associated with an increase in blood flow. The animal in panel C did not develop occlusive thrombus during the observation time period due to repeated embolization. (D-E) Neither the 7E9-treated mouse (D) nor the γΔ5 mouse (E) developed an occlusive thrombus during the 30-minute period.
