Close-up images of representative 2-DE gel images showing changes in signaling proteins that have been identified by differential analysis. (A) ILK-2. (B) Pleckstrin. (C) RKIP. (D) RGS10. B indicates 2-DE gel corresponding to basal platelets; and T, 2-DE gel corresponding to TRAP-activated platelets (5 μM, 30 s). Circled areas indicate the differentially regulated protein feature.