K562 cells stably transfected with FucT-VII exhibit E-selectin-mediated growth inhibition and apoptosis in the absence of PSGL-1. (A) Adhesion of K562 alone or transfected with PSGL-1 (KP cells), FucT-VII (KF cells), or both (KPF cells) to microwells coated with P-selectin (1800 sites/μm²) or E-selectin (550 sites/μm²). (B) Proliferation of K562, KP, KF, and KPF cells grown in serum-deprived medium on microwells coated with BSA, P-selectin, or E-selectin. Cells were seeded at 10⁴/mL and counted after 5 days incubation. (C) Apoptosis of K562, KP, KF, and KPF cells grown in serum-deprived medium on microwells coated with 10μg/mL BSA, P-selectin, or E-selectin. TUNEL assay was performed after 5 days incubation. *indicates statistically different (P < .05) from BSA control groups. Results are expressed as mean ± SEM of 3 independent experiments made in triplicate.