Figure 10.
The PD169540 ErbB inhibitor has no effect on the growth of the hematopoietic progenitors or on the adhesion of MM cells to stromal cells. (A) Bone marrow mononuclear cells from 5 patients with MM were grown in a semisolid culture medium with hematopoietic cytokines for 14 days. Cells were treated with either the PD169540 pan-ErbB inhibitor (1μM) or anti–IL-6 MoAb (B-E8; 10 μg/mL) or 10–6 M dexamethasone (DEX), alone or in combination. The number of granulocyte-macrophage colonies was counted on day 14 of incubation. Data are means ± SD of the number of colonies determined on 2 different culture plates for each condition. (B) XG-11 cells were cultured with either BMSC-coated (▪) or noncoated culture plates (□), without or with 1μM PD169540 for 24 hours. Nonadherent cells were counted and adherent cells were trypsinized and counted. In the 2 cell populations, GFP+ stromal cells were quantified with FACS analysis. Results are expressed as percentage of bound plasma cells ± SDs. The percentage was calculated as follows: percentage bound cells = ([input cells] – [nonadherent cells]/[input cells]) × 100. Results are of one experiment representative of 3.

The PD169540 ErbB inhibitor has no effect on the growth of the hematopoietic progenitors or on the adhesion of MM cells to stromal cells. (A) Bone marrow mononuclear cells from 5 patients with MM were grown in a semisolid culture medium with hematopoietic cytokines for 14 days. Cells were treated with either the PD169540 pan-ErbB inhibitor (1μM) or anti–IL-6 MoAb (B-E8; 10 μg/mL) or 10–6 M dexamethasone (DEX), alone or in combination. The number of granulocyte-macrophage colonies was counted on day 14 of incubation. Data are means ± SD of the number of colonies determined on 2 different culture plates for each condition. (B) XG-11 cells were cultured with either BMSC-coated (▪) or noncoated culture plates (□), without or with 1μM PD169540 for 24 hours. Nonadherent cells were counted and adherent cells were trypsinized and counted. In the 2 cell populations, GFP+ stromal cells were quantified with FACS analysis. Results are expressed as percentage of bound plasma cells ± SDs. The percentage was calculated as follows: percentage bound cells = ([input cells] – [nonadherent cells]/[input cells]) × 100. Results are of one experiment representative of 3.

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