Forced expression of C/EBPα leads to growth arrest and cell cycle arrest in 32D cells expressing FLT3/ITD. (A) A quantity of 1 × 106 32D/α-ER/FLT3/ITD and 32D/FLT3/ITD cells were cultured in the absence or presence of estradiol (1 μM) for 3 days. Viable cells were counted daily on the basis of trypan blue exclusion. Results shown are the means from triplicate assays. Error bars indicate SD. (B) The 32D/α-ER/FLT3/ITD and 32D/FLT3/ITD cells were incubated with estradiol for 24 hours. Cells (1 × 106) were collected before and after exposure to estradiol, treated with 20 μg/mL RNase, and stained with 50 μg/mL propidium iodide. Cells were subjected to flow cytometric analysis of DNA content. Data represented were from 1 of 3 different experiments.
