Expression of tissue factor on the surface of infected endothelial cells. Endothelial cell monolayers grown on glass coverslips were incubated for 8 hours with tissue culture medium (A), LPS (B), or germ tubes of A fumigatus (C-D). The expression of tissue factor on the endothelial cell surface was detected by confocal microscopy using an anti–tissue factor monoclonal antibody (A,C). Panel D shows the differential interference contrast image corresponding to the same field as panel C. Results are representative of 3 independent experiments. Original magnification × 400.