Accumulation of Homer-3 in the nucleus following TCR stimulation. Jurkat cells were stimulated with OKT3 (5 μg/mL) for 0, 10, 60, or 240 minutes at 37°C, and then nuclear extract was obtained as described in “Materials and methods.” The nuclear extract (15 μg of total proteins per well) was resolved on a 7.5% SDS-PAGE gel and blotted with anti-p65 antibody or anti–Homer-3 antibody. As already known, p65 accumulated in the nucleus after TCR stimulation. The amount of Homer-3 was also increased in the nucleus at 10 minutes after TCR stimulation and then decreased gradually. ^* indicates nonspecific bands with anti-p65 antibody.