Nuclear translocation of EVH1 domain fragment in Jurkat cells stimulated with anti-CD3 antibody. Jurkat cells were electroporated with Myc construct expressing EVH1 domain or coiled-coil domain (CCD) fragment of Homer-3. Twenty-four hours later, the cells were incubated for 10 minutes at 37°Con glass slides coated with poly-l-lysine only or poly-l-lysine and OKT3 and then stained with anti-Myc antibody. Both fragments were detected in the cytoplasmic region after incubation on poly-l-lysine–coated glass slides. After stimulation with OKT3, the EVH1 domain fragment was detected in the nucleus, whereas the CCD fragment formed cytoplasmic aggregates. Representative images were presented from experiments performed at least 3 times. Bars, 10 μm.