American Society of Hematology

Figure 1.

$Figure 1. IgG secretion by cultured B cells from 12 HIV-1–infected subjects. Unfractionated PBMCs were cultured at a concentration of 1 × 106 cells/mL. In the Transwell culture, B-cell–depleted PBMCs were seeded in the lower chamber and purified B cells were seeded in the upper chamber at the same ratio as in the unfractionated PBMCs. The corresponding amount of B cells was cultured with only medium. Supernatants were collected after 7 days. Results are expressed as mean ± SEM.$

IgG secretion by cultured B cells from 12 HIV-1–infected subjects. Unfractionated PBMCs were cultured at a concentration of 1 × 10⁶ cells/mL. In the Transwell culture, B-cell–depleted PBMCs were seeded in the lower chamber and purified B cells were seeded in the upper chamber at the same ratio as in the unfractionated PBMCs. The corresponding amount of B cells was cultured with only medium. Supernatants were collected after 7 days. Results are expressed as mean ± SEM.

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