Differentiation of mMSCs in culture. mMSCs were incubated to confluency in CEM and then transferred to adipogenic or osteogenic medium for 21 days. Chondrogenesis was produced by incubating 200 000 mMSCs as a micromass pellet in chondrogenic media for 21 days. All mMSCs were passage 7, except GFPtg cells, which were passage 4. Original magnification, × 10.