Schematic for the isolation of mMSCs. (A) The ends of each tibia or femur were removed, and the bones were placed in a pipet tip within a centrifuge tube. The cells were pelleted by centrifugation for 1 to 2 minutes at 400g. (B) Representative t-CFU and sc-CFU analysis. (C) The culturing steps for isolation and expansion of mMSCs.