Figure 3.
![Figure 3. Specificity of the CTL responses induced by DCs transfected with CLL-derived RNA. The specificity of CTLs induced with RNA-transduced DCs was analyzed by using autologous DCs from patient no. 1 pulsed with HLA-A2 binding peptides derived from the adipophilin, survivin, MUC1 and telomerase tumor-associated antigens as targets (A). Autologous DCs electroporated with EGFP mRNA were used as a negative control. Survivin-specific IFN-γ production was determined in an IFN-γ ELISPOT assay (B). The number of spots was determined by using an automatic ELISPOT reader (C). Values represent number of spots per 2 × 105 T cells after subtraction of spots counted in wells stimulated with unpulsed PBMNCs (dimethyl sulfoxide [DMSO] control).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/103/5/10.1182_blood-2003-06-2097/6/zh80050457280003.jpeg?Expires=1723418129&Signature=aJ372wakp8a3gFucshbjR8ANsaI5wXy4iS4rU8whtN1KIXWYIXcLiKB25vtOVpHCo~lpMRv0zbHoqWrTxtKcpYglMMffA6b8RKMM7WTVbWUlyvv7~Iu-AYEsg7Q~mL89vgOexhNF~pv9OeNSNtITMDfr6xQ2ChNXXf4eWozemqhtypP3UsJcan15DLhSCfXSi47qhsZR5B82TeBMbtIEtt7IgDLoWfypia1xa8j-iEEC4O1TGOqlqZCG4fWRqnzP56dQ0PMI6SjUC8W4GQ4h0C2VdX4D7oPudcfKcGqFdrZz1VzOA92ODQbHfVgUpW6Hkvxq8svPD3tk12QN5-N1yg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Specificity of the CTL responses induced by DCs transfected with CLL-derived RNA. The specificity of CTLs induced with RNA-transduced DCs was analyzed by using autologous DCs from patient no. 1 pulsed with HLA-A2 binding peptides derived from the adipophilin, survivin, MUC1 and telomerase tumor-associated antigens as targets (A). Autologous DCs electroporated with EGFP mRNA were used as a negative control. Survivin-specific IFN-γ production was determined in an IFN-γ ELISPOT assay (B). The number of spots was determined by using an automatic ELISPOT reader (C). Values represent number of spots per 2 × 105 T cells after subtraction of spots counted in wells stimulated with unpulsed PBMNCs (dimethyl sulfoxide [DMSO] control).
