Micrographs of a micropipette-aspirated, PE-Ter119–labeled reticulocyte. (A) Bright field illumination of a micropipette ready to aspirate a multilobular, young reticulocyte. (B) Bright field illumination of aspirated reticulocyte showing that the multilobular structure of the reticulocyte membrane could be smoothed out by aspirating the cell into the micropipette. (C) Fluorescence microscopy of the aspirated PE-Ter119–labeled reticulocyte showed a density gradient of GPA along the deformation projection. For scale reference, the diameter of the micropipette is 1.5 μm.