Involvement of pp60^c-src on the proliferation of a patient-derived ALCL cell line. The ALCL cell line KARPAS was transfected or not by a specific pp60^c-src siRNA or a control siRNA and seeded at 3 × 10⁴ cells per well in a 96-well plate. At 24 hours after transfection, cell proliferation was determined each day for 2 days using an MTT proliferation assay. Reported results are mean ± SEM of at least 4 experiments. The decrease in proliferation rate observed in the presence of pp60^c-src siRNA compared with control siRNA or untransfected cells was significant according to Student t test (P < .01). The expression level of pp60^c-src and actin was assessed by immunoblotting 48 hours after transfection.