Figure 5.
![Figure 5. Effects of inhibitory antibodies and receptor antagonists on Ca2+ responses of platelets interacting with collagen under flow. Blood was spiked with 10% Fluo-3–loaded autologous platelets, incubated with antagonists, and perfused over collagen, as indicated for Figure 1. Changes in [Ca2+]i were recorded during perfusion in single collagen-adherent platelets. Traces above are averaged curves from 15 to 25 platelets (3 donors); traces below are representative curves from single platelets. (A) Control. (B) 10B12 at 50 μg/mL. (C) 10B12 at 300 μg/mL. (D) 1C3 at 100 μg/mL. (E) 6F1 at 20 μg/mL. (F) GFOGER-GPP at 500 μg/mL. (G) ADP-receptor and TxA2 blockade, as in Figure 1.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/103/4/10.1182_blood-2003-03-0889/6/zh80040456790005.jpeg?Expires=1722455880&Signature=3d3vjbtyXPxJ0s8zG37nkpBecG--g2zcQ4e-l4WZ01ksts4nCAab9DjLkJ1ibAHfbdoZqz7jV1nLTmCa9i8ZIlld~obcGAjQcKfARBDkcT07t3nP6BHxxuiozv0tu67Dz7LvRoLNaxsJn1KrInh4pVUrzg~HeSx~Vk8xfMm-2EPvOof5LGTaQUxTgvYS23anhzPwCAKHB2amuGzHiw4ggFjN-D4-CNuSJnRt3cop8ceGeAjBa7nA4kBvpBJdhRMYkq5mFX3HT54y7cE8pK5qvgLLB6V3vkcz-HOaHPau1bS01WcHAWsQKcvT7x4Vg~XTDwKzbfHA~HBfF0LHps7irQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effects of inhibitory antibodies and receptor antagonists on Ca2+ responses of platelets interacting with collagen under flow. Blood was spiked with 10% Fluo-3–loaded autologous platelets, incubated with antagonists, and perfused over collagen, as indicated for Figure 1. Changes in [Ca2+]i were recorded during perfusion in single collagen-adherent platelets. Traces above are averaged curves from 15 to 25 platelets (3 donors); traces below are representative curves from single platelets. (A) Control. (B) 10B12 at 50 μg/mL. (C) 10B12 at 300 μg/mL. (D) 1C3 at 100 μg/mL. (E) 6F1 at 20 μg/mL. (F) GFOGER-GPP at 500 μg/mL. (G) ADP-receptor and TxA2 blockade, as in Figure 1.
