Figure 1.
Effects of inhibitory antibodies and receptor antagonists on thrombus formation. Images (120 × 120 μm) were recorded using a phase-contrast microscope after the perfusion of PPACK-anticoagulated whole blood over a collagen-coated surface for 4 minutes at a shear rate of 1000 s–1 Antibodies or inhibitors were added 15 minutes before perfusion. (A) Control. (B-D) 10B12 at 50, 100, or 300 μg/mL, respectively. (E) scFv 1C3 at 100 μg/mL. (F) 6F1 at 20 μg/mL. (G) GFOGER-GPP at 500 μg/mL. (H) ADP-receptor and TxA2 blockade by 40 μM MRS2179, 20 μM AR-C69931MX, 1 U/mL apyrase, and 100 μM ASA. Images shown are representative of 3 to 9 independent experiments. Collagen fibers are visible in some of the panels. Original magnification, × 60.
