EPCs coexpressing CD133 and KDR in proliferating hemangiomas. Hematopoietic cells were removed from a single cell suspension prepared from proliferating hemangioma specimen 75 using anti-CD45 magnetic microbeads. Before (top) and after (bottom) depletion, cells were double-labeled with PE- or FITC-conjugated mouse antihuman isotype-matched IgG controls, or PE-conjugated anti-CD133 and FITC-conjugated anti-CD45, or PE-conjugated anti-CD133 and FITC-conjugated anti-KDR. Labeled cells were analyzed by flow cytometry and 20 000 events were acquired. Percentage of cell population expressing single or double markers are shown in each quadrant.