Chemokine-induced Ca²⁺ mobilization differs in MoDCs matured in the presence or absence of PGE2. MoDCs stimulated with sCD40L in the absence (A-B) or presence (C-D) of PGE2 were loaded with fluo-3-AM, and chemokine-induced Ca²⁺ mobilization was analyzed by flow cytometry. Baseline was established for 30 seconds before chemokines (250 ng/mL) were added, as indicated by the arrow. Ionomycin (1 μg/mL) was added (A-B) to ensure proper fluo-3-AM loading of MoDCs. Representative results obtained from MoDCs of a single donor are shown. At least 5 independent experiments with MoDCs from different donors yielded similar results.