Expression of Hsp70L1. (A) Northern blot analysis of Hsp70L1 tissue distribution, using full-length Hsp70L1 cDNA as a probe. Molecular size markers are indicated. (B) Expression levels of Hsp70L1 mRNA in various cell lines, as determined by semiquantitative RT-PCR. (C) Inducible expression of Hsp70L1 mRNA in HeLa cells. HeLa cells were pretreated with heat, TNF-α, or PMA, then analyzed by RT-PCR for Hsp70L1 mRNA expression. (D) Expression of Hsp70L1 mRNA in DCs. DCs cultured for 6 days were stimulated with the indicated stimuli, then RT-PCR analysis of Hsp70L1 expression performed.