Figure 5.
![Figure 5. Effect of autoantibody adsorption with immobilized GPIIb-IIIa. Biotinylated murine monoclonal anti-GPIIb (2A9), coupled to avidin-Sepharose beads, was incubated with normal platelet lysate to allow GPIIb-IIIa binding. After washing, test plasmas (patient plasmas containing anti-GPIIb/IIIa antibody [ITP-1, ITP-5] and control plasmas [Cont-1, Cont-2]) were incubated with 2 separate aliquots of the immobilized GPIIb-IIIa, followed by centrifugation. Adsorption removed more than 80% of the autoantibody, as demonstrated in binding assays. Unadsorbed and adsorbed (ads) plasmas were tested, and the total numbers of megakaryocytes present after 10 days were determined.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/103/4/10.1182_blood-2003-08-2672/6/zh80040456710005.jpeg?Expires=1724214467&Signature=pQO2R4Z-i-AzTtgCVCrnJi9Td0Lev981uQ8ZaQQMbV5cbWmuwolmfX8sWbHDOvGKOJlc5S-QZlCWh-j60ZxxQa7-Wp6DkkR8fedDWBFU2uJxmjzEp1vP0LiTGt0QQSktgCRvlyNH8JTH52UlO7HFWLOTwHDQR2WSnajzEraDvqU0-tMwf8EeMnngevqzJZHlOVgdN04yPWfh4AIKGoVdcqQMRQ9Ptp3G~DU4DKj66rLk-7Z5IktMjtzjTPilMTxmcSMUpmXRPvLRDRo0p08rMDiswP3zsfvJcU6bVh4Irm~chMLlaO0kT3mW7OwZRaGu7pDesWc2dz1Doxp8etL86Q__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of autoantibody adsorption with immobilized GPIIb-IIIa. Biotinylated murine monoclonal anti-GPIIb (2A9), coupled to avidin-Sepharose beads, was incubated with normal platelet lysate to allow GPIIb-IIIa binding. After washing, test plasmas (patient plasmas containing anti-GPIIb/IIIa antibody [ITP-1, ITP-5] and control plasmas [Cont-1, Cont-2]) were incubated with 2 separate aliquots of the immobilized GPIIb-IIIa, followed by centrifugation. Adsorption removed more than 80% of the autoantibody, as demonstrated in binding assays. Unadsorbed and adsorbed (ads) plasmas were tested, and the total numbers of megakaryocytes present after 10 days were determined.
