Figure 3.
Figure 3. Suppression of megakaryocyte production by ITP plasma dilutions. CD34+-rich cells were cultured in media containing rHuMGDF and serially diluted plasma from patient ITP-1. After harvesting the cultures on day 10, the total number of megakaryocytes was determined [cell number (determined by direct counting of each culture) × % megakaryocytes (determined from FACS data)]. % Control megakaryocytes = mean total megakaryocytes in ITP cultures/mean total megakaryocytes in control cultures × 100.

Suppression of megakaryocyte production by ITP plasma dilutions. CD34+-rich cells were cultured in media containing rHuMGDF and serially diluted plasma from patient ITP-1. After harvesting the cultures on day 10, the total number of megakaryocytes was determined [cell number (determined by direct counting of each culture) × % megakaryocytes (determined from FACS data)]. % Control megakaryocytes = mean total megakaryocytes in ITP cultures/mean total megakaryocytes in control cultures × 100.

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